Dye-nucleoprotein Interactions in Giemsa Banding

Fixed chromosome preparations subjected to treatments such as pH 9 (I) or trypsin hydrolysis (2) and stained with Giemsa's demonstrate similar banding patterns irrespective of the pretreatments used. It has been shown that this phenomenon is the result of modifications of DNA-protein and protein-protein associations which alter dye interaction (3-5). It appears that only Giemsa's or related staining solutions can produce the characteristic banding after appropriate treatment (6), and therefore, the present study was designed to identify those corn-